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| This is part of my studies at Stephen F. Austin State University for my Masters in Biotechnology. This is a brief overview of my research and some protocols for the various parts of my project. |
| An Extensive Survey of Rumex crispus for Anittumor Compounds |
| Rumex crispus is a common weed that grows all over the world. It has been used as an herbal remedy for centuries. It is commonly used to treat skin ailments and as a laxative. R. crispus also has a high iron content, therefore it is used to treat anemia and as a "blood cleanser" There is also some evidence that this ubiquitous plant may have antitumor properties. |
| My study of this plant will utilize solvent extracts from five parts of the plant: rhizome, leaf, root, stem, and flower. Each of these extracts will then be further extracted using solid phase extraction to separate potential compounds into their chemical classes for ease in identification of possible new antitumor compounds. |
| The extracts will then be exposed to colon cancer cells in a 96-well plate at 10 fold dilutions to generate a dose-response curve for samples that demonstrate cell death. Positive control of cell death will be used (doxorubicin) as well as a control with a normal colon cell line to test toxicity on normal cells of the antitumor compounds. |
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